Improved Method to Retrieve DNA from Dried Silver-stained Polyacrylamide Gels

Improved method to retrieve DNA from dried silver-stained polyacrylamide gels.

absence of MTX. Only a minor increase of 12.7% 6 9.3% (1.1 6 0.8 mmol/L) was observed 8 h after the injection, a diurnal change that is in the same range as previously reported in healthy individuals (18). In contrast, there was a variable but substantial increase in tHcy in patients after MTX infusion, reaching a maximum of 49.3% 6 61.6% (3.0 6 2.4 mmol/L) after 12 h, demonstrating the antifol...

Western blot of stained proteins from dried polyacrylamide gels.

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

Proteins from silver-stained gels can be digested enzymatically and the resulting peptides analyzed and sequenced by mass spectrometry. Standard proteins yield the same peptide maps when extracted from Coomassieand silver-stained gels, as judged by electrospray and MALDI mass spectrometry. The low nanogram range can be reached by the protocols described here, and the method is robust. A silver-...

Efficient cloning of DAF polymorphic markers from silver-stained polyacrylamide gels.

DNA amplification fingerprinting (DAF) (1) gives about 40–60 bands per polyacrylamide gel lane, which makes the polymerase chain reaction (PCR) pattern rather complex for band isolation from a gel. The presence of minor amplified bands having only a few base pairs difference from major bands interferes with the product cloning because of co-isolation (3). This fact becomes obvious if a multiply...

Coloration of silver-stained protein bands in polyacrylamide gels is caused by light scattering from silver grains of characteristic sizes.

This study investigates the physical basis of color effects in the detection of proteins in polyacrylamide gels by silver staining. Specifically, the hypothesis that different colors may correlate with the development of silver grains of characteristic sizes was investigated by electron microscopy. Protein bands that stained brown, yellow, and blue were excised from stained gels and prepared fo...